Facile preparation of pH-sensitive biocompatible alginate beads havening layered double hydroxide supported metal-organic framework for controlled release from doxorubicin to breast cancer cells

In the present study, a pH-sensitive controlled drug delivery system was prepared from alginate beads impreg-nated with Al-Cu layered double hydroxides (LDH) supported copper metal-organic framework (Alg-DOX-Cu MOF-LDH beads) for controlled release from doxorubicin (DOX) to breast cancer cells. The physicochemical properties of Alg-DOX-Cu MOF-LDH beads were determined by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), field emission scanning electron microscopy (FE-SEM), and Zeta potential. The in vitro release studies showed that the Alg-DOX-Cu MOF-LDH beads had more controlled drug release than the DOX-Cu MOF-LDH and in vitro release of DOX from both carriers showed pH-response release behavior. The kinetic adsorption studies also confirmed that the drug release from the DOX-Cu MOF-LDH and Alg-DOX-Cu MOF-LDH beads well obey the Korsmeyer-Peppas model. Meanwhile, in vitro cytotoxicity and DAPI tests on L929 (non-cancerous cells) and MCF-7 (human breast cancer cells) clearly showed that the prepared Al-Cu LDH and Cu MOF-LDH systems had good biocompatibility. Whereas, Alg-DOX-Cu MOF-LDH beads had higher cytotoxicity effects as a result of the controlled release of DOX to MCF-7 cells. These results suggested that the Alg-DOX-Cu MOF-LDH beads can be used as an excellent drug delivery system for cancer therapy and other biomedical applications.

Automated summary

In this study, a pH-sensitive drug delivery system using Alginate beads impregnated with Al-Cu layered double hydroxides supported copper metal-organic framework was prepared for controlled release of doxorubicin to breast cancer cells. The physicochemical properties of the beads were analyzed, and in vitro release studies showed controlled drug release with pH-response. Cytotoxicity tests showed that the Alg-DOX-Cu MOF-LDH beads had higher cytotoxicity effects on breast cancer cells.