Physicochemical properties and protective effects on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides by fractional precipitation

The development of natural ingredients protecting skin from UVA-induced photoaging is widely expected. The present study investigated the physicochemical properties, antioxidant, moisturizing, collagenase and elastase inhibitory activities, and protective effect on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides (POPs). POP-40, POP-60, and POP-80 were extracted by gradient precipitation of 40 %, 60 %, and 80 % ethanol, which could be prepared in large quantities. The results showed that POPs had good DPPH and ABTS radical scavenging abilities, water retention capacity, and collagenase and elastase inhibition effects. POP -80 had the best efficacy. Further determined the anti-inflammatory and antisenescence activities of POPs in Hs68 cells. The results indicated that after UVA irradiation, the contents of ROS, senescent cells, NF-cB activity, and proinflammatory cytokines increased in Hs68 cells. However, cells pretreated with 50 mu g/mL POPs significantly decreased the contents of ROS and the number of senescent cells, reduced NF-cB activity, and inhibited IL-6 and TNF-alpha production. There was no significant difference in reducing the accumulation of ROS and senescent cells between POP-80 and the common anti-inflammatory substance quercetin. The results suggested that POP-80 may be potential cosmeceutical ingredients as it can protect Hs68 cells from photodamage.

Automated summary

The polysaccharides extracted from Pleurotus ostreatus showed good antioxidant, moisturizing, collagenase and elastase inhibition activities, and protective effect on UVA-induced photoaging in Hs68 cells. Among them, POP-80 exhibited the best efficacy. These findings suggest that POP-80 may be a potential cosmeceutical ingredient for protecting skin from photodamage.