期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 189, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2022.115748
关键词
Gluconic acid; Enzymatic hydrolysate; Fermentation; Gluconobacter oxydans (G. oxydans)
资金
- National Natural Science Foundation of China [31901270]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [SJCX22_0324]
- Qing Lan Project of Jiangsu Province, China
- Start-up funds of Nanjing Forestry University for Scientific Research [163030163]
- Advanced Analysis and Testing Center of Nanjing Forestry University
Efficiency of gluconic acid production from cellulosic materials can be improved through a combined strategy of activated carbon treatment and high-tension oxygen supply, enabling industrial-scale production.
Cellulosic hydrolysate from the hydrolysis of glucan-riched biomass can offer abundantly bioavailable glucose, which is an alternative feedstock for gluconic acid production. In order to speed up the process of cellulosic-based gluconic acid synthesis industrialization, cost reduction by using efficient substrate in batch-fermentation with high yield and productivity is extremely important. However, enriched glucose obtained by concentrating enzymatic hydrolysate inevitably ascends the viscosity, resulting in a lower oxygen transfer rate that cannot meet the requirement of bio-oxidation with Gluconobacter oxydans, resulting in gluconic acid productivity decreased drastically once the glucose content in the concentrated enzymatic hydrolysate was over 240 g/L with corresponding viscosity over 4 mPa center dot s. In view of the above problems, a combined strategy of activated carbon treatment and high-tension oxygen supply was applied to improve efficiency. Finally, approximately 230 g/L gluconic acid with 9.6 g/L/h productivity was achieved, which enabled the industrial-scale gluconic acid bioproduction from cellulosic materials.
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