Comparison of polyol-based deep eutectic solvents (DESs) on pretreatment of moso bamboo (Phyllostachys pubescens) for enzymatic hydrolysis

Four polyol-based deep eutectic solvents (DESs) were designed for bamboo pretreatment and enzymatic hydrolysis, and the pretreatment conditions including temperature, time and acid dosage in the optimized DES were comprehensively evaluated. The results showed that the components separation and saccharification of bamboo could be promoted by polyol-based DESs pretreatment, which are positively correlated with the hydroxyl contents of hydrogen bond donor in DESs, except for sorbitol-based DES. Excessive hydroxyl contents could lead to the increase of DES viscosity and lignin deposition, which had a negative effect on saccharification. Meanwhile, the optimum pretreatment conditions in xylitol-based DES (ChCl/XL) were determined to be 120 degrees C for 3 h with 1wt% acid, and 96.08% of glucose yield was recorded. The recycling experiment indicated that ChCl/XL retained acceptable pretreatment performance. This work gives novel insights into the design and selection of polyol-based DES for bamboo pretreatment and enzymatic hydrolysis.

Automated summary

This study designed polyol-based deep eutectic solvents (DESs) for bamboo pretreatment and enzymatic hydrolysis, and comprehensively evaluated the pretreatment conditions. The results showed that polyol-based DESs can promote the separation of components and saccharification of bamboo. Additionally, a xylitol-based DES was determined to have the optimal pretreatment conditions, with good recyclability performance.