期刊
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS II-EXPRESS BRIEFS
卷 69, 期 11, 页码 4513-4517出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TCSII.2022.3184749
关键词
Sensors; Microfluidics; Temperature sensors; Heating systems; Temperature measurement; CMOS technology; Microelectrodes; Electrowetting-on-dielectric (EWOD); microelectrode dot array (MEDA); digital microfluidics biochip (DMFB); bio-protocol; programmable biochip
资金
- MOE in Taiwan under AiM for High-Value Creation Project
- MOST in Taiwan [108-2221-E-009-071-MY3]
The digital lab-on-CMOS chip for nucleic acid amplification test features novel circuit design and precise microfluidic operations, enabling it to meet various medical testing requirements while reducing reagent consumption and test time/cost.
A digital lab-on-CMOS chip is presented for nucleic acid amplification test. This biochip consists of programmable microelectrode dot array and digital timing controller. With novel circuit design in each microelectrode, microfluidic operations with velocity of 0.6mm/s, temperature profile with maximum change rate of 4.5 degrees C/s and location sensing in mu second-level can be achieved. As a result, test samples can be located first, followed by target operations to meet the requirements of specific medical tests with less reagent consumption and test time/cost. Furthermore bio-protocols can be derived to ensure both replicability and reliability in various medical tests. Preliminary results in loop-mediated isothermal amplification (LAMP) and PCR thermal cycle tests demonstrate the feasibility of our proposal with less test time of <40 min and <1 min respectively, making our proposal very suitable for mobile healthcare applications.
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