期刊
GLIA
卷 71, 期 5, 页码 1233-1246出版社
WILEY
DOI: 10.1002/glia.24334
关键词
cell proliferation; optic nerve head astrocyte; Piezo1; YAP
Optic nerve head (ONH) astrocytes play a crucial role in supporting neuronal axons through their mechanical sensitivity, mediated by the ion channel Piezo1. Knockdown or overexpression of Piezo1 in vitro revealed its necessity but insufficiency in ONH astrocyte proliferation. Loss of Piezo1 led to cell cycle arrest, possibly through decreased YAP nuclear localization and downregulation of cell cycle-associated factors. Gene ontology analysis highlighted the impact of Piezo1 on cell division processes. These findings establish Piezo1 as an essential regulator of cell cycle progression in ONH astrocytes.
Optic nerve head (ONH) astrocytes provide structural and metabolic support to neuronal axons in developmental, physiological, and pathological progression. Mechanosensitive properties of astrocytes allow them to sense and respond to mechanical cues from the local environment. We confirmed that ONH astrocytes express the mechanosensitive ion channel Piezo1 in vivo. By manipulating Piezo1 knockdown or overexpression in vitro, we found that Piezo1 is necessary but insufficient for ONH astrocyte proliferation. Loss of Piezo1 can lead to cell cycle arrest at G0/G1 phase, a possible mechanism involving decreased yes-associated protein (YAP) nuclear localization and downregulation of YAP-target cell cycle-associated factors, including cyclin D1 and c-Myc. Gene ontology enrichment analysis of differential expression genes from RNA-seq data indicates that the absence of Piezo1 affects biological processes involving cell division. Our results demonstrate that Piezo1 is an essential regulator in cell cycle progression in ONH astrocytes.
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