Bacterial community analysis of infant foods obtained from Chinese markets by combining culture-dependent and high-throughput sequence methods

In this study, twenty-two baby foods including cereal-based products and powdered infant formula (PIF) ob-tained from local markets were comprehensively investigated for their bacterial contamination using culture -dependent and high-throughput sequence (HTS) methods. In addition, the genetic diversity and biofilm-forming capacity of the most abundant species were analyzed using random amplified polymorphic DNA (RAPD) and crystal violet staining assay, respectively. Results showed that 170 mesophilic isolates collected from 22 samples were clustered into 15 genera and 41 species. Bacillus (77.65%) was the most prevalent genus, fol-lowed by Paenibacillus (7.06%), Alkalibacillus (3.53%), and Lysinibacillus (2.35%). Bacillus licheniformis (49.41%) proved to be the most dominant species in infant foods, and a high genetic diversity with six different RAPD profiles was observed. A total of 87.5% of B. licheniformis isolates were identified as strong biofilm formers, and heterogeneous biofilm-forming ability was observed among the isolates sharing the same RAPD pattern. HTS analysis revealed an 18-fold higher biodiversity at the genus level, and a significantly different bacterial com-munity of infant foods was dominated by Lactococcus, Streptococcus, and Bifidobacterium. Foodborne pathogens including Bacillus cereus, and potentially pathogenic microorganisms such as Acinetobacter baumannii, were identified in infant foods by HTS. The current results could expand the crucial information about bacterial contamination of baby foods.

Automated summary

This study comprehensively investigated bacterial contamination in 22 baby foods using culture-dependent and high-throughput sequence methods, revealing Bacillus as the most prevalent genus. High-throughput sequencing identified significantly higher biodiversity and different bacterial communities in infant foods, expanding crucial information on bacterial contamination of baby foods.