In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model

beta-carotene is a carotenoid with provitamin A activity and other health benefits, which needs to become bioavailable upon oral intake to exert its biological activity. A better understanding of its behaviour and stability in the gastrointestinal tract and means to increase its bioavailability are highly needed. Using an in vitro gastrointestinal digestion method coupled to an intestinal cell model, we explored the stability, gastrointestinal bioaccessibility and cellular uptake of beta-carotene from microparticles containing carotenoid extracts derived from mango by-products. Three types of microparticles were tested: one with the carotenoid extract as such, one with added inulin and one with added fructooligosaccharides. Overall, beta-carotene was relatively stable during the in vitro digestion, as total recoveries were above 68 %. Prebiotics in the encapsulating material, especially inulin, enhanced the bioaccessibility of beta-carotene almost 2-fold compared to microparticles without prebiotics. Likewise, beta-carotene bioaccessibility increased proportionally with bile salt concentrations during digestion. Yet, a bile salts level above 10 mM did not contribute markedly to beta-carotene bioaccessibility of prebiotic containing microparticles. Cellular uptake experiments with non-filtered gastrointestinal digests yielded higher absolute levels of beta-carotene taken up in the epithelial cells as compared to uptake assays with filtered digests. However, the proportional uptake of beta-carotene was higher for filtered digests (24 - 31 %) than for non-filtered digests (2 - 8 %). Matrix-dependent carotenoid uptake was only visible in the unfiltered medium, thereby pointing to possible other cellular transport mechanisms of non-micellarized carotenoids, besides the concentration effect. Regardless of a filtration step, inulin-amended microparticles consistently resulted in a higher beta-carotene uptake than regular microparticles or FOS-amended microparticles. In conclusion, encapsulation of carotenoid extracts from mango by-products displayed chemical stability and release of a bioaccessible beta-carotene fraction upon gastrointestinal digestion. This indicates the potential of the microparticles to be incorporated into functional foods with provitamin A activity.

Automated summary

Beta-carotene, a carotenoid with provitamin A activity, shows stability and release of bioaccessible fraction upon gastrointestinal digestion in microparticles containing carotenoid extracts from mango by-products. The addition of prebiotics, especially inulin, enhances the bioaccessibility of beta-carotene. Cellular uptake experiments indicate higher absolute levels of beta-carotene in epithelial cells for non-filtered gastrointestinal digests, while filtered digests show a higher proportional uptake of beta-carotene. In conclusion, the encapsulation of carotenoid extracts in microparticles has the potential to be incorporated into functional foods with provitamin A activity.