Rapid quantitative detection of Vibrio parahaemolyticus via high-fidelity target-based microfluidic identification

With the rapid development of logistics, a growing number of pathogenic microorganisms has the means to spread worldwide using food as a carrier; thus, there is an urgent need to develop effective detection strategies to ensure food safety. By combining novel markers identified by pan-genome analysis and a digital recombinase-aided amplification (RAA) detection method based on a microfluidic chip, a strategy of high-fidelity target -based microfluidic identification (HFTMI) has been developed. Herein, a proof-of-concept study of HFTMI for rapid pathogen detection of V. parahaemolyticus was investigated. Specific primers designed for the gene group_41170 identified in the pan-genome analysis showed high sensitivity and a broad spectrum for the detection of V. parahaemolyticus. Different power systems were investigated to increase the partition rate on specifically designed chamber-based digital chips. The performance of HFTMI was greatly improved compared with qPCR. Collectively, this novel HFTMI system provides more reliable guidance for food safety testing.

Automated summary

With the rapid development of logistics, there is an urgent need to develop effective detection strategies to ensure food safety, as a growing number of pathogenic microorganisms spread worldwide using food as a carrier. This study developed a high-fidelity target-based microfluidic identification (HFTMI) strategy by combining novel markers identified by pan-genome analysis and a digital recombinase-aided amplification (RAA) detection method. The HFTMI system showed improved performance compared to qPCR, providing more reliable guidance for food safety testing.