Isolation and functional characterization of hemp seed protein-derived short- and medium-chain peptide mixtures with multifunctional properties for metabolic syndrome prevention

This study aims to obtain a valuable mixture of short-chain peptides from hempseed as a new ingredient for developing nutraceutical and functional foods useful for preventing metabolic syndrome that represents the major cause of death globally.A dedicated analytical platform based on a purification step by size exclusion chromatography or ultrafil-tration membrane and high-resolution mass spectrometry was developed to isolate and comprehensively char-acterize short-chain peptides leading to the identification of more than 500 short-chain peptides. Our results indicated that the short-chain peptide mixture was about three times more active than the medium-chain peptide mixture and total hydrolysate with respect to measured inhibition of the angiotensin-converting enzyme. The short-chain peptide mixture was also two times more active as a dipeptidyl peptidase IV, and twofold more active on the cholesterol metabolism pathway through the modulation of low-density lipoprotein receptor.

Automated summary

This study aims to develop a valuable mixture of short-chain peptides from hempseed for the prevention of metabolic syndrome worldwide. A dedicated analytical platform was developed to isolate and characterize over 500 short-chain peptides, which showed higher activity in inhibiting angiotensin-converting enzyme and dipeptidyl peptidase IV, and modulating cholesterol metabolism through low-density lipoprotein receptor.