期刊
FOOD MICROBIOLOGY
卷 109, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2022.104142
关键词
Antifungal proteins; Sliced bread; Fungal spoilage; Shelf life; Control
This study aimed to isolate and identify fungal species involved in sliced bread spoilage, and to evaluate their susceptibility to antifungal proteins of fungal origin. The study found that Penicillium species were the predominant mycobiota, with P. roqueforti, P. brevicompactum, P. chrysogenum and P. crustosum being the most abundant species. PdAfpB and PeAfpA were the most potent proteins in inhibiting fungal growth, while PeAfpC was the least active protein.
This study aimed to isolate and identify fungal species involved in sliced bread spoilage, and to evaluate their susceptibility to antifungal proteins of fungal origin (AFPs). Proteins include PdAfpB from Penicillium digitatum and PeAfpA, PeAfpB and PeAfpC from Penicillium expansum. Based on morphological criteria, a group of sixteen fungal isolates were selected and subsequently identified at the species level using sequence analysis. Penicillium species, the predominant mycobiota, were identified based on a combined phylogenetic analysis using ITS and beta-tubulin sequences, being P. roqueforti, P. brevicompactum, P. chrysogenum and P. crustosum the most abundant species. Aspergillus versicolor, Aspergillus niger and Bissochlamys spectabilis were also identified. Regarding the antifungal activity of AFPs, PdAfpB and PeAfpA were the most potent proteins since the growth of most of tested fungi was completely inhibited by concentrations ranging from 2 to 32 mu g/mL. PeAfpB showed moderate anti -fungal activity, whereas PeAfpC was the least active protein. The best in vitro AFPs, PdAfpB and PeAfpA, were also evaluated in in situ protection assays against P. roqueforti. PdAfpB provoked a clear reduction of P. roqueforti growth in sliced bread samples, suggesting that this AFP has a protective effect on bread. This study underlines the potential of the AFPs tested, in particular PdAfpB, as alternative antifungal agents for extending sliced bread shelf life.
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