Simple and reliable determination of B group vitamins in various food matrices with the use of the voltammetric sensor based on Ni-zeolite/carbon black nanocomposite

In this work, a low-cost and highly selective voltammetric method for the determination of vitamin B-2 (VB2), B-9 (VB9), B-12 (VB12), and B3 (VB3) with the use of Ni-zeolite/carbon black modified glassy carbon electrode (NiZCB-GCE) is presented. Through the combination of specific properties of the zeolite and carbon black, the proposed sensor showed an enhancement effect on reduction signals of studied vitamins. The NiZCB-GCE exhibited linear response in the range of 0.008-0.24 mg L-1, 0.004-0.22 mg L-1, 0.003-0.1 mg L-1, and 0.15-10 mg L-1 with the limit of detection equal to 2.3 mu g L-1, 1.3 mu g L-1, 0.92 mu g L-1, and 0.045 mg L(-1 )obtained for VB2, VB9, VB12, and VB3, respectively. The developed protocol was successfully applied for the direct determination of the above-mentioned vitamins in dietary supplements, multivitamin juices and energy drinks, and food products, i.e., breakfast cereals and instant cocoa drinks without laborious sample preparation.

Automated summary

A low-cost and highly selective voltammetric method for the determination of vitamins B-2, B-9, B-12, and B3 using a Ni-zeolite/carbon black modified glassy carbon electrode was presented. The proposed sensor showed an enhancement effect on reduction signals of the studied vitamins. The developed protocol was successfully applied for the direct determination of the above-mentioned vitamins in dietary supplements, multivitamin juices and energy drinks, and food products without laborious sample preparation.