期刊
FOOD CHEMISTRY
卷 396, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133729
关键词
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资金
- National Key Research and Development Program of China [2016YFD0401204]
- Key Area R & D Program of Guangdong Province [2019B020211002]
- Guangdong Basic and Applied Basic Research Foundation [2021B1515120042]
- Natural Science Foundation of Guangdong Province [2021A1515012422]
- Guangzhou Planned Program in Science and Technology [202102021002]
- Research Fund Program of Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology [KF2021004]
In this work, a specific monoclonal antibody against tyramine was produced using a new hapten design. A high-resolution multicolor colorimetric immunoassay for tyramine was developed by integrating enzyme-induced multicolor generation with smartphone-assisted signal readout. The immunoassay showed a limit of detection of 19.7 mg/kg or L in beef, pork, and yogurt, and the results were reliable.
In this work, a specific monoclonal antibody against tyramine was produced based on a new hapten design. Then, we developed a high-resolution multicolor colorimetric immunoassay for tyramine based on this antibody by integrating enzyme-induced multicolor generation with smartphone-assistant signal readout. The multicolor generation is due to the shift of the local surface plasmon resonance band of gold nanostructure controlled by alkaline phosphatase-induced the growth of gold nanostars. Quantitative detection of tyramine was achieved via analyzing the red/blue channel values of assay solution's image taken by a smartphone with the support of a color recognizer application. The limit of detection of this immunoassay for tyramine detection in beef, pork and yoghurt was 19.7 mg/kg or L. The average recoveries were between 83 % and 103 %., and the results were validated by high performance liquid chromatography to be reliable. Overall, this developed immunoassay provides a promising platform for on-site detection of tyramine.
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