4.7 Article

Discovery of a novel phosphotransferase from Bacillus subtilis that phosphorylates a broad spectrum of flavonoids

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FOOD CHEMISTRY
卷 400, 期 -, 页码 -

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ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.134001

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Bacillus subtilis; Biosynthesis; Flavonoid; Phenolic compounds; Phosphorylation; Phytochemicals

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Flavonoid O-phosphorylation provides a potent modification to enhance the oral bioavailability of flavonoids. The enzyme BsFPS, discovered in this study, efficiently catalyzes the phosphorylation of structurally-diverse flavonoids. The findings highlight the potential for developing biosynthetic platforms to obtain new phosphorylated flavonoids for pharmaceutical and nutraceutical applications.
Flavonoids are associated with health benefits, but most of them have poor oral bioavailability due to their extremely low aqueous solubility. Flavonoid O-phosphorylation suggests a potent modification to solve the problems. Here, we isolated, identified and characterized an unprecedented phosphotransferase, flavonoid phosphate synthetase (BsFPS), from B. subtilis. The enzyme catalyzes the ATP-dependent phosphorylation of flavonoid to generate flavonoid monophosphates, AMP and orthophosphate. BsFPS is a promiscuous phospho-transferase that efficiently catalyzes structurally-diverse flavonoids, including isoflavones, flavones, flavonols, flavanones and flavonolignans. Based on MS and NMR analysis, the phosphorylation mainly occurs on the hy-droxyl group at C-7 of A-ring or C-4 ' of B-ring in flavonoid skeleton. Notably, BsFPS is regioselective for the ortho-3 ',4 '-dihydroxy moiety of catechol-containing structures, such as luteolin and quercetin, to produce phosphate conjugates at C-4 ' or C-3 ' of B-ring. Our findings highlight the potential for developing biosynthetic platform to obtain new phosphorylated flavonoids for pharmaceutical and nutraceutical applications.

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