期刊
FOOD CHEMISTRY
卷 396, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133664
关键词
Pleurotus eryngii; Selenium biofortification; Bioaccessibility; Gut microbiota; Lead adsorption
资金
- National Natural Science Foundation of China [32072213]
The development of safe and efficient dietary selenium sources is crucial for improving lead toxicity. This study investigated the biofortification and bioaccessibility of selenium in Pleurotus eryngii by supplying selenate, selenite, SeMet, and Se-yeast. It was found that Se-enriched P. eryngii, particularly cultivated with Se-yeast, had higher organic selenium content and bioaccessibility. Furthermore, these Se-enriched mushrooms promoted the growth of Desulfovibrio, leading to increased lead adsorption by gut microbiota.
It is of great significance to develop safe and efficient dietary selenium sources to improve lead toxicity. In this study, selenate, selenite, SeMet and Se-yeast were supplied to investigate the Se biofortification and bioaccessibility in Pleurotus eryngii. The effects of Se-enriched P. eryngii on lead binding bacteria were investigated via in vitro fermentation. With 40 mg/kg Se in the substrate, the total Se contents of P. eryngii treated with selenite and Se-yeast were 145.22 +/- 8.00 mg/kg and 90.01 +/- 7.01 mg/kg, respectively. Compared with selenite, Se-yeast treatment significantly increased the organic Se proportion in P. eryngii (SeCys2 2.85 +/- 0.17%, MeSeCys 2.33 +/- 0.21% and SeMet 78.19 +/- 1.58%), which led to higher bioaccessibility. With 1 mg/L lead treatment during in vitro fermentation, Se-enriched P. eryngii promoted the growth of Desulfovibrio, which contributed to the increase of gut microbiota lead adsorption. Se-enriched P. eryngii cultivated with Se-yeast could be used as dietary Se sources for lead toxicity improvement.
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