Lipase-catalyzed production of pyridoxine monolaurate in solvent-free bioreactor system

This study demonstrated that solvent-free gas bubbling system enhanced production efficiency of pyridoxine monolaurate in the esterification catalyzed by immobilized Candida antarctica lipase B (Lipozyme 435). Volumetric productivity in solvent-free gas bubbling system (41.24 mmol/L/h) was 3.7 and 2.1-fold higher than those in conventional organic solvent system (11.10 mmol/L/h) and solvent-free system (19.86 mmol/L/h) using magnetic stirring, respectively. Among the three bioreactor systems, solvent-free gas bubbling system provided the best reusability of the lipase retaining 94.45 % of initial activity for six batch reactions. In the bioreactor system, 5-O-lauroyl-pyridoxine was regioselectively produced with maximum production of 371.17 mmol/L at 70 ? and 0.10 of substrate molar ratio ([pyridoxine]/[lauric acid]) for 9 h. Pyridoxine monolaurate exhibited interfacial activity at oil-water interfaces, suggesting it had emulsifying properties. Pyridoxine monolaurate is expected to be applied as a multi-functional emulsifier with nutritional values to replace both small molecule emulsifiers and pyridoxine hydrochloride in fortified beverages.

Automated summary

This study demonstrates that the production efficiency of pyridoxine monolaurate can be enhanced by using a solvent-free gas bubbling system with immobilized Candida antarctica lipase B. Moreover, this system shows the best reusability of the lipase and the highest volumetric productivity compared to conventional organic solvent and solvent-free systems.