4.7 Article

Transcriptome analysis of hepatopancreas in penaeus monodon under acute low pH stress

期刊

FISH & SHELLFISH IMMUNOLOGY
卷 131, 期 -, 页码 1166-1172

出版社

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.11.031

关键词

Penaeus monodon; Low pH stress; Transcriptome; Hepatopancreas

资金

  1. China Agriculture Research System of MOF and MARA [CARS-48]
  2. Central Public Interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS [2020ZD01, 2021SD13]
  3. Hainan Provincial Naturl Science Foundation of China [320QN359, 322RC806, 320LH008]
  4. Guangdong Basic and Applied Basic Reuter Foundation [2020A1515110200]
  5. Guangzhou Science and Technology Planning Project [202102020208]
  6. Hainan Yazhou Bay Seed Laboratory [B21Y10701, B21HJ0701]

向作者/读者索取更多资源

The decrease of seawater pH can negatively affect the metabolism, acid-base balance, immune response, and immunopro-tease activity of aquatic animals. In this study, differentially expressed genes related to lipid metabolism, induction of oxidative stress, and inflammatory response in the muscle of penaeus monodon under low pH stress were identified through high-throughput sequencing analysis. These genes provide valuable reference information for future breeding of low pH-tolerant shrimp.
The decrease of seawater pH can affect the metabolism, acid-base balance, immune response and immunopro-tease activity of aquatic animals, leading to aquatic animal stress, impairing the immune system of aquatic animals and weakening disease resistance, etc. In this study, we performed high-throughput sequencing analysis of the hepatopancreas transcriptome library of low pH stress penaeus monodon, and after sequencing quality control, a total of 43488612-56271828 Clean Reads were obtained, and GO annotation and KEGG pathway enrichment analysis were performed on the obtained Clean Reads, and a total of 395 DEGs were identified. we mined 10 differentially expressed and found that they were significantly enriched in the Metabolic pathways (ko01100), Biosynthesis of secondary metabolites (ko01110), Nitrogen metabolism (ko00910) pathways, such as PIGA, DGAT1, DGAT2, UBE2E on Metabolic pathways; UGT, GLT1, TIM genes on Biosynthesis of secondary metabolites; CA, CA2, CA4 genes on Nitrogen metabolism, are involved in lipid metabolism, induction of oxidative stress and inflammation in the muscular body of spot prawns. These genes play an important role in lipid metabolism, induction of oxidative stress and inflammatory response in the muscle of the shrimp. In summary, these genes provide valuable reference information for future breeding of low pH-tolerant shrimp.

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