期刊
FISH & SHELLFISH IMMUNOLOGY
卷 131, 期 -, 页码 342-348出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.10.020
关键词
Silver pomfret; Photobacterium; Transcriptome; Infection; Gene expression
资金
- National Natural Science Foundation of China [42106105]
- Zhejiang Provincial Natural Science Foundation of China [LQ21C190002, LQ20C190004]
- Ningbo Public Welfare Science and Technology Project [2021S061]
- Ningbo Natural Science Foundation [202003N4121]
- Ningbo 2025 Major Project of Science Technology and Innovation [2021Z003]
- Ningbo Yongjiang Talent Introduction Programme [2021B-029-C]
- Fundamental Research Funds for the Provincial Universities of Zhejiang
- K. C. Wong Education Foundation
This study conducted transcriptome analysis of silver pomfret infected with Photobacterium damselae subsp. damselae (PDD) to investigate the molecular mechanisms of host defense. Differentially expressed genes and immune-related pathways were identified, providing valuable information for strategies against Photobacterium spp. infection in teleosts.
Silver pomfret has been widely cultured in China due to its high economic value. Photobacterium damselae subsp. damselae (PDD) is a Gram-negative bacterium that has been shown to infect many fish species. To increase knowledge of the molecular mechanisms of the host defense against PDD, we conducted transcriptome analysis of head kidney in silver pomfret at 24 h and 72 h post-infection (hpi) via Illumina sequencing. The de novo assembly resulted in the identification of 79,063 unigenes, with 59,386 (75.11%) successfully annotated in public data-bases (NR, NT, KO, Swiss-Prot, Pfam, GO, and KOG databases). Comparison of gene expression profiles between PBS-injected fish (sham control) and PDD-challenged fish revealed 329 and 570 differentially expressed genes (DEGs) were screened at 24 hpi and 72 hpi, respectively. The DEGs were enriched in multiple immune-related pathways such as Hepatitis C, Gastric acid secretion, CAMs and Leukocyte transendothelial migration path-ways, Primary immunodeficieny, ECM-receptor interaction, PI3K-Akt signaling pathway. The data obtained in the present study offers valuable information for acute immune response of silver pomfret challenged with PDD, which will facilitate further investigations on strategies against Photobacterium spp. infection in teleosts.
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