4.7 Article

Ischemic stroke rehabilitation through optogenetic modulation of parvalbumin neurons in the contralateral motor cortex

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EXPERIMENTAL NEUROLOGY
卷 360, 期 -, 页码 -

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ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.expneurol.2022.114289

关键词

GABA; Glu; Motor function; Optogenetic; Parvalbumin neurons; Interhemispheric inhibition; Ischemic stroke

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Optogenetic stimulation can improve the excitation/inhibition balance between bilateral motor cortices, leading to reduced neurological deficit and improved motor dysfunction in ischemic stroke rats.
Background: Based on the theory of interhemispheric inhibition and the bimodal balance-recovery model in stroke, we explored the effects of excitation/inhibition (E/I) of parvalbumin (PV) neurons in the contralateral primary motor cortex (cM1) connecting the ipsilateral M1 (iM1) via the corpus callosum (cM1-CC-iM1) of ischemic stroke rats by optogenetic stimulation.Methods: We tested this by injecting anterograde and retrograde virus in rats with middle cerebral artery oc-clusion (MCAO), and evaluated the neurological scores, motor behavior, volume of cerebral infarction and the E/ I balance of the bilateral M1 two weeks after employing optogenetic treatment.Results: We found that concentrations of Glu and GABA decreased and increased, respectively, in the iM1 of MCAO rats, and that the former increased in the cM1, suggesting E/I imbalance in bilateral M1 after ischemic stroke. Interestingly, optogenetic stimulation improved M1 E/I imbalance, as illustrated by the increase of Glu in the iM1 and the decrease of GABA in both iM1 and cM1, which were accompanied by an improvement in neurological deficit and motor dysfunction. In addition, we observed a reduced infarct volume, an increase in the expression of the NMDAR and AMPAR, and a decrease in GAD67 in the iM1 after intervention.Conclusions: Optogenetic modulation of PV neurons of the iM1-CC-cM1 improve E/I balance, leading to reduced neurological deficit and improved motor dysfunction following ischemic stroke in rats.

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