Gastric cancer-derived mesenchymal stem cells promote gastric cancer cell lines migration by modulating CD276 expression

CD276 has been studied in a variety of cancers and diseases, but its regulatory mechanisms in gastric cancer is still unclear. Mesenchymal stem cells (MSCs), one of the important members of tumor microenvironment, play an important role in the occurrence, development and metastasis of tumor, but the relationship between gastric cancer mesenchymal stem cells (GCMSCs) and CD276 in gastric cancer needs to be further explored. The differential expression of CD276 was identified via UCLAN and GEPIA databases. Then, the impacts of CD276 were calculated on clinical prognosis using the Kaplan-Meier plotter and Cox analysis. GO, KEGG and GSEA analysis were used to explore potential mechanism under CD276. Next, the expression of CD276 in gastric cell lines were detected by Western blot. Immunocoprecipitation was used to explore the association between CD276 and COL1A1. And the effect of condition medium (CM) from GCMSCs on gastric cell lines migration analyzed. GC-MSCs activated the AKT/c-Myc/mTOR pathway of gastric cell lines and upregulated CD276 expression. Moreover, the upregulation of CD276 promoted the migration of gastric cancer cells. Taken together, this study shown that GCMSCs could up-regulate the expression of CD276 of gastric cell lines to promote tumor migration. Our results provide a new basis for the treatment of gastric cancer.

Automated summary

CD276 has been studied in various cancers and diseases, but its regulatory mechanisms in gastric cancer remain unclear. Mesenchymal stem cells (MSCs), important members of the tumor microenvironment, play a significant role in tumor occurrence, development, and metastasis. However, the relationship between gastric cancer mesenchymal stem cells (GCMSCs) and CD276 needs further exploration. This study found that GCMSCs up-regulated the expression of CD276 in gastric cell lines to promote tumor migration, providing a new basis for the treatment of gastric cancer.