Acupuncture Alleviates Corneal Inflammation in New Zealand White Rabbits with Dry Eye Diseases by Regulating α7nAChR and NF-κB Signaling Pathway

Purpose. The purpose of this study is to determine the mechanism of improvement in dry eye diseases (DEDs) treated by acupuncture. The inflammatory molecules and related pathways will be analyzed in our study. Methods. In order to establish the animal model for DEDs, healthy New Zealand white rabbits were treated with scopolamine (Scop) hydrobromide for 21 consecutive days. After 21 days, acupuncture, fluorometholone (Flu), and alpha 7nAChR antagonist (alpha-BGT) treatments were performed, and the Scop injections were continued until day 35. The therapeutic effect of acupuncture on DED inflammation was evaluated by corneal fluorescence staining, tear film rupture time, tear flow measurement, in vivo confocal microscopy (IVCM), corneal histopathology, and cytokine protein chip technology. The influence of acupuncture on the corneal pathology and inflammatory factors ACh, alpha 7nAChR, and NF-kappa B was detected by enzyme-linked immunosorbent assay (ELISA) and western blot. Results. Compared with the group Scop, acupuncture can significantly reduce corneal staining and increase the tear film rupture time and tear flow, which are accompanied by a decrease in corneal epithelial detachment and lymphocyte infiltration. Acupuncture can relieve the inflammation of corneal stroma and mitigate the expression of proinflammatory factors and chemokines. Acupuncture can upregulate the expression of ACh and alpha 7nAChR and downregulate the expression of NF-kappa B. Conclusion. Our findings demonstrate that acupuncture can alleviate corneal inflammation in New Zealand white rabbits with DEDs via alpha 7nAChR and NF-kappa B signaling pathway regulation. The expression indicates that alpha 7nAChR/NF-kappa B signaling pathway may be active and that acupuncture is a potential therapeutic target for dry eye.

Automated summary

This study investigates the mechanism of improvement in dry eye diseases (DEDs) treated by acupuncture. The results demonstrate that acupuncture can alleviate corneal inflammation and reduce corneal damage and inflammatory cell infiltration in DEDs. Acupuncture achieves this by regulating the expression of ACh, alpha 7nAChR, and NF-kappa B, suggesting that acupuncture may be a potential therapeutic target for dry eye.