期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 97, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.etap.2022.104042
关键词
EE2; Endocrine disruption; Vitellogenin; Zona pellucida; Aromatase; Wap
Currently, endocrine disruptors (EDs) can be found in all the environmental compartments. The effects of acute exposure to 17 alpha-ethinylestradiol (EE2) in C. variegatus larvae were investigated. The results showed that EE2 exposure at different concentrations affected the gene expression of various markers, indicating that C. variegatus larvae are an efficient and sensitive model for biomonitoring estrogenic EDs (EEDs).
Currently, endocrine disruptors (EDs) can be found in all the environmental compartments. To understand the effects of estrogenic EDs (EEDs), adults of Cyprinodon variegatus have been classically used as a marine model. However, it is during development that exposure to contaminants may generate permanent consequences. Thus, the aim of this study was to verify the effects produced by acute exposure to 17 alpha-ethinylestradiol (EE2) in C. variegatus larvae. Quantitative PCR (qPCR) results revealed the induction of vtg and zp gene expression on exposure to 1000 ng/L EE2 and the induction of vtgc, zp2, zp3 and cyp19a2, and inhibition of vtgab, wap and cyp1a1 on exposure to 100 ng/L EE2. Lower concentrations inhibited the gene expression of vtgab and wap (50 ng/L), cyp1a1 (25 ng/L) and zp2 (12.5 ng/L). These alterations in gene expression allow us to affirm that larvae of C. variegatus are an efficient and sensitive model for biomonitoring EEDs.
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