期刊
ENVIRONMENTAL TOXICOLOGY
卷 38, 期 4, 页码 820-832出版社
WILEY
DOI: 10.1002/tox.23726
关键词
apoptosis; eucalyptol; immune function; oxidative stress; tetrabromobisphenol A
In this experimental study, the mechanism of TBBPA toxicity on Grass carp hepatocytes (L8824 cells) and the antagonistic impact of EUC on TBBPA were investigated. The results showed that TBBPA exposure induced oxidative stress and activated the ASK1/JNK signaling pathway, resulting in apoptosis. Additionally, EUC effectively protected against TBBPA-induced apoptosis and immune dysfunction by regulating the ROS/ASK1/JNK pathway.
Tetrabromobisphenol A (TBBPA) is a common environmental pollutant which has multi-organ toxicity to mammals. Eucalyptol (EUC) has super antioxidant biological activity. However, in this experimental study, we probed into the mechanism of toxic of TBBPA exposure on Grass carp hepatocytes (L8824 cells) and the antagonistic impact of EUC on TBBPA. We treated L8824 cells with 8 mu g/ml TBBPA and/or 20 mu M EUC for 24 h in this test research. The experiment results suggested that TBBPA exposure induced elevated levels of reactive oxygen species (ROS), led to oxidative stress, decreased SOD and CAT activities, decreased GSH and T-AOC contents, exacerbated MDA accumulation, activated ASK1/JNK signaling pathway, and further increased the contents of mitochondrial dependent apoptosis pathway related indicators (Cyt-C, Bax, Caspase 9, Caspase 3), while Bcl-2 expression decreased. In addition, TBBPA exposure induced increased expression of TNF-alpha, IL-6, IL-1 beta, and decreased expression of IL-2, IFN-gamma, Hepcidin, beta-defensin, LEAP2. The oxidative stress level, ASK1/JNK signal pathway expression level, apoptosis ratio and cellular immune function of cells exposed to EUC alone did not change significantly. Combined exposure of TBBPA and EUC significantly reduced the proportion of apoptosis and restored cellular immune function. Therefore, these results suggest that EUC can effectively antagonize TBBPA-induced apoptosis and immune dysfunction of L8824 cells by regulating ROS/ASK1/JNK signaling pathway.
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