4.4 Article

Immobilized enzyme with sustainable chestnut biochar to remediate polycyclic aromatic hydrocarbons contaminated soils

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ENVIRONMENTAL TECHNOLOGY
卷 -, 期 -, 页码 -

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TAYLOR & FRANCIS LTD
DOI: 10.1080/09593330.2022.2164221

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White rot fungus; immobilized crude enzymes; chestnut-based biochar; polycyclic aromatic hydrocarbons; benzo[a]pyrene

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In this study, acid-modified chestnut inner shell biochar was used to immobilize white-rot fungal crude enzyme for the remediation of PAHs-contaminated soil. The maximum immobilization rate of crude enzyme reached 97.25% under optimal conditions. Immobilization enhanced the stability of the crude enzyme, with a relative activity increase of 59.32% and 49.73% after 5 weeks of storage at 4 degrees C and 25 degrees C, respectively. The chestnut-based immobilized crude enzyme successfully degraded 37% of benzo[a]pyrene in 10 days for PAHs-contaminated soils. Overall, this study explores an efficient, feasible, and low-cost remediation method for PAHs-contaminated soils and provides technical support for the application of crude enzymes in organic contaminated soils.
Polycyclic aromatic hydrocarbons (PAHs) contaminated soil severely and are difficult to remediate. In this study, acid-modified chestnut inner shell biochar with abundant pore channels was used as the main raw materials for the immobilization of white-rot fungal crude enzyme. The maximum immobilization rate of crude enzymes (97.25%+/- 6.20%) could be achieved under the optimal conditions of 24 h immobilization of 10 U/mL crude enzymes by 1 g biochar at 25celcius and pH = 5. Meanwhile, immobilization improved the stability of the crude enzyme. The relative activity of the immobilized crude enzyme increased by 59.32% and 49.73% (compared to the free crude enzymes) after 5 weeks of storage at 4 degrees C and 25 degrees C, respectively. It has been verified that chestnut-based immobilized crude enzyme can degrade 37% of benzo[a]pyrene in 10 days for PAHs-contaminated soils. An efficient, feasible, and low-cost remediation method for PAHs-contaminated soils was explored, which provides technical support for the application of crude enzymes in organic contaminated soils.

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