Bio-functionalized copper oxide/chitosan nanocomposite using Sida cordifolia and their efficient properties of antibacterial, anticancer activity against on breast and lung cancer cell lines

Nanoparticles synthesis from green chemistry method is gaining a lot of attention due to their non-toxic, low cost and facile. In this study, a copper oxide nanoparticle (CuO NPs) was synthesized using Sida cordifolia aqueous leaf extract and incorporated chitosan biomolecules to potential enhancing of biological properties. The CuO NPs and chitosan (CS) embedded nanocomposite was noted as CuO-CS nanocomposite, its was physicochemical char-acterized by using of UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray Diffraction (XRD) and Field emission scanning electron microscopy (FE-SEM) with Energy dispersive X-ray (EDX) analysis. Bio-functionalized CuO-CS nanocomposite was performed antibacterial efficiency against both Gram positive (Staphylococcus aureus, Bacillus subtilis) and Gram negative (Salmonella typhi, Escherichia coli) bacteria through the Mueller Hinton agar (MHA) well diffusion techniques. The highest bactericidal activity was revealed Gram positive of B. subtilis and Gram negative of S. typhi bacteria, respectively. Further, the cytotoxicity effect of biosynthesized nanocomposite was an examined against human breast cancer MDA-MB-231 and lung cancer A549 cell lines. The half maximal inhibitory concentration is showed at 2 mu g/mL for MDA-MB-231and 4 mu g/mL was A549 cells. Live/dead cells were detected by fluorescence microscopic observation at the IC50 concentra-tion. In furthermore, bio-functionalized CuO-CS nanocomposite was performed photocatatlytic dye degradation against for industrial dyes of crystal violet (CV) and malachite green (MG). From the results, synergic bio-functionalized CuO-CS nanocomposite was suggested potential suitable for biomedical applications as well as industrial wastewater treatment.

Automated summary

In this study, CuO-CS nanocomposite was synthesized using Sida cordifolia aqueous leaf extract and was found to possess antimicrobial activity against both Gram-positive and Gram-negative bacteria. It also exhibited cytotoxic effects on breast cancer and lung cancer cells. Additionally, the nanocomposite showed efficient photocatalytic dye degradation, suggesting its potential applications in biomedical and industrial wastewater treatment.