Rare earth element scandium mitigates the chromium toxicity in Lemna minor by regulating photosynthetic performance, hormonal balance and antioxidant machinery

Chromium (Cr) toxicity is a serious problem that threatens the health of living organisms and especially agri-cultural production. The presence of excess Cr leads to biomass loss by causing the imbalance of biochemical metabolism and inhibiting photosynthetic activity. A new critical approach to cope with Cr toxicity is the use of the rare earth elements (REEs) as an antioxidant defence system enhancer in plants. However, the effect of scandium (Sc), which is one of the REEs, is not clear enough in Lemna minor exposed to Cr toxicity. For this purpose, the photosynthetic and biochemical effects of scandium (50 mu M and 200 mu M Sc) treatments were investigated in Lemna minor under Cr stress (100 mu M, 200 mu M and 500 mu M Cr). Parameters related to photo-synthesis (Fv/Fm, Fv/Fo) were suppressed under Cr stress. Stress altered antioxidant enzymes activities and hormone contents. Sc applications against stress increased the activities of superoxide dismutase (SOD), NADPH oxidase (NOX), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and glutathione S-transferase (GST). In addition to the antioxidant system, the contents of indole-3-acetic acid (IAA), abscisic acid (ABA) and jasmonic acid (JA) were also rearranged. However, in all treatment groups, with the provision of ascorbate (AsA) regeneration and effective hormone signaling, reactive oxygen species (ROS) retention which result in high hydrogen peroxide (H2O2) content and lipid peroxidation (TBARS) were effectively removed. Sc promoted the maintenance of cellular redox state by regulating antioxidant pathways included in the AsA-GSH cycle. Our results showed that Sc has great potential to confer tolerance to duckweed by reducing Cr induced oxidative damage, protecting the biochemical reactions of photosynthesis, and improving hormone signaling.

Automated summary

Chromium (Cr) toxicity is a serious problem that negatively affects living organisms and agricultural production. The use of scandium (Sc) as an antioxidant enhancer has shown potential for mitigating the harmful effects of Cr toxicity in plants. This study investigated the effects of Sc treatments on Lemna minor exposed to Cr stress, and found that Sc increased antioxidant enzyme activities and hormone levels, while reducing oxidative damage and improving photosynthesis.