4.7 Article

Polybrominated diphenyl ethers interact with the key protein involved in carbohydrate metabolism in rice

期刊

ENVIRONMENTAL POLLUTION
卷 316, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2022.120466

关键词

Polybrominated diphenyl ethers; Rice; Soluble sugar; Biomass; Photoaffinity probe; Chemoproteomics

向作者/读者索取更多资源

This study revealed the mechanism of how polybrominated diphenyl ethers (PBDEs) affect rice, and identified fructose-1, 6-bisphosphate aldolase (FBA) as the target protein of 2, 2 ', 4, 4 '-tetrabromodiphenyl ether (BDE47). It combined novel chemoproteomics with conventional proteomics to investigate the direct interaction between organic pollutants and proteins.
Rice exposed to organic pollutants such as polybrominated diphenyl ethers (PBDEs) usually experiences reduced biomass and increased soluble sugar content. This study showed that 2, 2 ', 4, 4 '-tetrabromodiphenyl ether (BDE47) led to increased glucose, fructose, and sucrose in rice leaves, accompanied by decreased photosynthetic rate and biomass. In order to identify the key enzyme that BDE-47 interacted with, a diazirine-alkynyl photoaffinity probe was designed, and photoaffinity labeling based chemoproteomics was conducted. Among all differentially expressed proteins, fructose-1, 6-bisphosphate aldolase (FBA) involved in carbohydrate metabolism was most likely the target protein of BDE-47. Spectral techniques and molecular docking analysis further revealed that the pollutant-protein interaction was driven by hydrophobic force. BDE-47 inhibited FBA catalytic efficiency by competing with its substrate, fructose-1, 6-diphosphate (F-1, 6-P), leading to soluble sugar accumulation, photosynthetic rate decline and biomass reduction. This study unraveled the influencing mechanism of PBDEs on rice by combining the novel photoaffinity labeling-based chemoproteomics with conventional proteomics. The improved knowledge on direct interaction between organic pollutants and proteins will help alleviate the harmful effects of soil pollution on plants.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据