期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 33, 期 -, 页码 8-14出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2016.03.013
关键词
Inflammatory pain; Cytokines; Oxidative stress; Leukocyte recruitment; NF-kappa B
资金
- Coordenadoria de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Sao Paulo Research Foundation, Ministerio da Ciencia, Tecnologia e Inovacao (MCTI) [2011/19670-0, 2013/08216-2]
- SETI
- Fundacao Araucaria
- Parana State Government
- CAPES/ Fundacao Araucaria
Lipopolysaccharide (LPS) is the major structural component of Gram-negative bacteria cell wall and a highly pro-inflammatory toxin. Naringenin is found in Citrus fruits and exhibits antioxidant and anti-inflammatory properties through inhibition of NF-kappa B activation but its effects in LPS-induced inflammatory pain and leukocyte recruitment were not investigated yet. We investigated the effects of naringenin in mechanical hyperalgesia, thermal hyperalgesia and leukocyte recruitment induced by intraplantar injection of LPS in mice. We found that naringenin reduced hyperalgesia to mechanical and thermal stimuli, myeloperoxidase (MPO, a neutrophil and macrophage marker) and N-acetyl-beta-D-glucosaminidase (NAG, a macrophage marker) activities, oxidative stress and cytokine (TNF-alpha, IL-1 beta, IL-6, and IL-12) production in the paw skin. In the peritoneal cavity, naringenin reduced neutrophil and mononuclear cell recruitment, and abrogated MPO and NAG activity, cytokine and superoxide anion production, and lipid peroxidation. In vitro, pre-treatment with naringenin inhibited superoxide anion and cytokine (TNF-alpha, IL-1 beta, IL-6, and IL-12) production by LPS-stimulated RAW 264.7 macrophages. Finally, we demonstrated that naringenin inhibited NF-kappa B activation in vitro and in vivo. Therefore, naringenin is a promising compound to treat LPS-induced inflammatory pain and leukocyte recruitment. (C) 2016 Elsevier Inc. All rights reserved.
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