期刊
EMU-AUSTRAL ORNITHOLOGY
卷 123, 期 1, 页码 79-84出版社
TAYLOR & FRANCIS AUSTRALIA
DOI: 10.1080/01584197.2022.2158476
关键词
ACTH challenge; assay validation; enzyme-immunoassay; faecal glucocorticoid metabolites; physiological stress
类别
Faecal glucocorticoid metabolite (fGCM) analysis is a non-invasive method for monitoring adrenocortical responses to stress. This study validates the use of enzyme-immunoassays (EIAs) to quantify fGCMs in two bird species. Results show species-specific suitability of EIAs and the time lag between stress initiation and peak fGCM concentration.
Faecal glucocorticoid metabolite (fGCM) analysis provides a non-invasive, feedback-free approach for monitoring adrenocortical responses to natural and anthropogenic stressors. The use of enzyme-immunoassays (EIAs) to quantify immunoreactive fGCMs has gained popularity in recent years but requires species-specific validation prior to first use. We conducted a pharmacological challenge with adrenocorticotropic hormone (ACTH) to determine whether changes in circulating glucocorticoids are reflected in fGCM, concentrations and therefore to validate excreta as a matrix for monitoring endocrine status in a southern African passerine, the White-browed Sparrow-weaver (Plocepasser mahali) and a columbid, the Laughing Dove (Spilopelia capensis). We tested the suitability of four EIAs to quantify fGCMs in 10 individuals of each species. Two of the EIAs, tetrahydrocorticosterone and 11-Oxoetiocholanolone II, detected significant elevations and were therefore most suitable for quantifying fGCMs in the White-browed Sparrow-weavers. In contrast, the 5 alpha-pregnane-3 beta, 11 beta, 21-triol-20-one EIA detected the highest elevations in fGCM concentrations in the Laughing Doves. The lag time between stressor initiation (ACTH injection) and the resulting peak fGCM concentrations was similar to 2 h in both species. The validations presented here open opportunities for monitoring physiological responses in free-ranging individuals and contribute to our knowledge of the EIAs suitable for non-invasive quantification of avian fGCM concentrations.
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