期刊
EMBO REPORTS
卷 24, 期 3, 页码 -出版社
WILEY
DOI: 10.15252/embr.202255683
关键词
embryonic stem cell differentiation; kinase; LIF-Stat3; Lin41; Pfkp
This study reveals an interaction between LIF-Stat3 signaling and the glycolytic enzyme Pfkp in the differentiation of murine ESCs into specific cell lineages. The repression of Pfkp by Stat3 maintains pluripotency in ESCs, and lifting this repression results in differentiation towards the ectodermal lineage. Pfkp acts as a protein kinase to phosphorylate Lin41, stabilizing its expression and promoting the binding and degradation of mRNAs encoding ectodermal specification markers.
Unveiling the principles governing embryonic stem cell (ESC) differentiation into specific lineages is critical for understanding embryonic development and for stem cell applications in regenerative medicine. Here, we establish an intersection between LIF-Stat3 signaling that is essential for maintaining murine (m) ESCs pluripotency, and the glycolytic enzyme, the platelet isoform of phosphofructokinase (Pfkp). In the pluripotent state, Stat3 transcriptionally suppresses Pfkp in mESCs while manipulating the cells to lift this repression results in differentiation towards the ectodermal lineage. Pfkp exhibits substrate specificity changes to act as a protein kinase, catalyzing serine phosphorylation of the developmental regulator Lin41. Such phosphorylation stabilizes Lin41 by impeding its autoubiquitination and proteasomal degradation, permitting Lin41-mediated binding and destabilization of mRNAs encoding ectodermal specification markers to favor the expression of endodermal specification genes. This provides new insights into the wiring of pluripotency-differentiation circuitry where Pfkp plays a role in germ layer specification during mESC differentiation.
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