Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces

The early detection of anti-asparaginase biomarker can facilitate timely modification of asparaginase chemotherapy, thereby avoiding serious complications. Herein we describe the preparation of a novel electrochemical biosensing interface for rapid detection of anti-asparaginase in the picomolar range (1-10 000 pM). Coimmobilization of ferrocene and asparaginase on a carbon interface (via diazonium grafting) facilitates transduction through attenuation of the surface-bound ferrocene redox couple. The limit of detection of 0.8 pM for this point-of-care applicable method compares favourably to that of traditional faradaic assaying (2.0 pM) where transduction occurs by the target blocking the diffusion of the solution redox probe [Fe(CN)(6)](3-/4-).

Automated summary

The early detection of anti-asparaginase biomarker is important for timely modification of asparaginase chemotherapy and avoiding complications. A novel electrochemical biosensing interface was prepared for rapid detection of anti-asparaginase in the picomolar range. The coimmobilization of ferrocene and asparaginase facilitated transduction through attenuation of the surface-bound ferrocene redox couple, with a limit of detection of 0.8 pM.