期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 247, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.114269
关键词
Cu; Zn; G; affinis; Acute exposure; Gene expression; Indicator species
资金
- Agricultural Science Research Institute of Lianshan Zhuang and Yao Autonomous County (Doctor Workstation of Guangdong Province) , Science and Technology Program of Guangz-hou [20212100006]
- China Scholarship Council [201908440582]
- Talent introduction special funds of South China Agricultural University [K16226]
Water pollution is a serious problem. This study investigated the toxic effects of Cu and Zn ions on G. affinis by analyzing the changes in gene expression. The results showed that Cu and Zn have strong toxic effects on G. affinis. Furthermore, the study identified different gene expression patterns in different tissues of G. affinis exposed to Cu and Zn ions.
Water pollution is an increasingly serious problem. Here, Cu and Zn ions were used as stress factors, and G. affinis served as a test organism. Fluorescence quantitative PCR was used to detect changes in the expression of anti-oxidant genes (SOD, GST, CAT), heat stress genes (Hsp70, Hsp90, Hspd1, Hsc70), and immune system-related genes (IL-1 fl, IL-8) in G. affinis exposed to Cu and Zn ions over time. To explore the toxic effects of Cu and Zn on G. affinis. The results showed that the 48 h LC50 concentrations of the heavy metals Cu and Zn to G. affinis were 0.17 mg/L and 44.67 mg/L, respectively. Within 48 h, with prolonged Cu exposure, the relative expression levels of the Hsp70, Hsp90, Hspd1, Hsc70, SOD, GST, and CAT genes in the gill tissue first showed a significant increase and then gradually decreased. Gene expression peaked between 9 and 36 h. The relative expression levels of SOD and GST genes in liver tissue showed a gradual decline. Within 48 h, with prolonged Zn exposure, the expression levels of SOD, CAT, and GST genes in G. affinis first increased and then fell before finally rising. The expression levels of IL-1 fl and IL-8 mRNA showed varying degrees of upward trends, and the expression of IL -8 was the highest for all gill tissue. To sum up, Cu and Zn have strong toxic effects on G. affinis, which makes it possible to use G. affinis as indicator organisms for aquatic environmental pollution.
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