期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 250, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.114491
关键词
Endocrine disruptors; Benzo(a)pyrene; Glyphosate; Mixture; Insect; Gene expression; Wastewater
Many priority pollutants are concentrated in the environment due to human activity. The effects of single and binary exposure have been widely explored, but the simultaneous presence of several pollutants in the environment needs to be considered. Using the model organism Drosophila melanogaster, we investigated the impact of multiple pollutants on post-embryonic development and gene expression patterns. Molecular biology analyses enhance pollution monitoring and should be incorporated in toxicological studies.
Many priority pollutants are concentrated in the environment due to human activity. Most are highly toxic to various organisms, including endocrine disruptors EDCs, aromatic polycyclic hydrocarbons PAHs, pesticides. While the effects of single and binary exposure have been widely explored, several pollutants can be simultaneously present at the same time in the environment, in in more or less polluted matrices. Effective pollution control requires the presence and sources of contamination to be identified. Previously we used Drosophila melanogaster to investigate metal pollution. Here, we re-used Drosophila to identify the biomarkers of pollution, and to determine if they can be used for specific types of pollution. Single and combined exposure of Bis(2ethylhexyl) phthalate (DEHP), bisphenol A, nonylphenol, benzo(a)pyrene, and glyphosate was investigated. The impact of these pollutants on post-embryonic development and the expression pattern of 38 molecular targets were examined using qPCR. During single exposure, different profiles were observed at the molecular level. In complex mixtures, the expression profile resembled that of bisphenol A. In contrast, relatively specific gene expression profiles were obtained for the effects of each pollutant separately. While direct pollutant-gene profiling remains difficult in mixtures, molecular biology analyses enhance pollution monitoring, and should be incorporated in toxicological studies.
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