期刊
DRUG DELIVERY
卷 30, 期 1, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10717544.2022.2162157
关键词
Resveratrol; PEGylated bilosome; Caco-2 cell permeation; anti SARS-CoV-2; docking
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to a global catastrophe, prompting the development of a safe and effective therapeutic system. Polyphenols such as resveratrol (RSV) have proven antiviral activity. However, RSV suffers from limited solubility and degradation in the gastrointestinal tract and liver, hindering its clinical use. PEGylated bilosomes (PBs) containing a PEGylated edge activator were proposed to enhance the permeability and bioavailability of RSV. The optimized formula (F5) showed improved dissolution behavior, increased cellular uptake, and complete in vitro suppression of SARS-CoV-2, suggesting it as a promising oral treatment for SARS-CoV-2 infection.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) predisposed to the emergence of worldwide catastrophe that impels the evolution of safe and effective therapeutic system. Polyphenols as resveratrol (RSV) exhibit a well evidenced antiviral activity. Unfortunately, like most phenolic nutraceuticals, RSV suffers from restrained solubility and massive degradation in GIT and liver which in turn prohibit its clinical use. Herein, PEGylated bilosomes (PBs) contain PEGylated edge activator along with the traditional components as (Span 60, cholesterol and bile salts) were proposed to boost both permeability and bioavailability of RSV. The investigation of the prominent effect of the diverse variables on the characteristics of the vesicles and picking of the optimum formula were conducted via construction of 2(3) factorial experiment. The appraisal of the formulae was conducted on the basis of entrapment efficiency percent (EE%), particle size (PS) and zeta potential (ZP). In addition, the spherical shaped optimal formula (F5) exhibited EE% of 86.1 +/- 2.9%, PS of 228.9 +/- 8.5 nm, and ZP of -39.8 +/- 1.3 mV. The sorted optimum formula (F5) exhibited superior dissolution behaviors, and boosted Caco-2 cells cellular uptake by a round 4.7 folds relative to RSV dispersion. In addition, F5 demonstrated a complete in vitro suppression of SARS-CoV-2 at a concentration 0.48 mu g/ml with 6.6 times enhancement in antiviral activity relative to RSV dispersion. The accomplished molecular modeling heavily provided proof for the possible interactions of resveratrol with the key residues of the SARS-CoV2 Mpro enzyme. Finally, F5 could be proposed as a promising oral panel of RSV for curation from SARS-CoV-2 infection.
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