Cre recombinase microinjection for single-cell tracing and localised gene targeting

Tracing and manipulating cells in embryos are essential to understand development. Lipophilic dye microinjections, viral transfection and iontophoresis have been key to map the origin of the progenitor cells that form the different organs in the post-implantation mouse embryo. These techniques require advanced manipulation skills and only iontophoresis, a demanding approach of limited efficiency, has been used for single-cell labelling. Here, we perform lineage tracing and local gene ablation using cell-permeant Cre recombinase (TAT-Cre) microinjection. First, we map the fate of undifferentiated progenitors to the different heart chambers. Then, we achieve single-cell recombination by titrating the dose of TAT-Cre, which allows clonal analysis of nascent mesoderm progenitors. Finally, injecting TAT-Cre to Mycnflo/flo embryos in the primitive heart tube revealed that Mycn plays a cell-autonomous role in maintaining cardiomyocyte proliferation. This tool will help researchers identify the cell progenitors and gene networks involved in organ development, helping to understand the origin of congenital defects.

Automated summary

Tracing and manipulating cells in embryos are essential to understand development. Lipophilic dye microinjections, viral transfection, and iontophoresis have been key techniques for mapping the origin of progenitor cells. Here, the researchers used cell-permeant Cre recombinase microinjection to perform lineage tracing and gene ablation. The tool will help identify cell progenitors and gene networks involved in organ development and understand the origin of congenital defects.