期刊
DEVELOPMENT
卷 150, 期 1, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.201366
关键词
Caenorhabditis elegans; bHLH; Neurogenesis; HLH-2; Daughterless; E protein
We investigated whether there are common mechanisms of neurogenesis throughout the entire nervous system of C. elegans. Our findings suggest that while distinct proneural class II bHLH factors play a role in neurogenesis, their function is not universal and is instead limited by lineage, cell type, and differentiation programs affected. Genetic removal of the hlh-2 gene activity showed broad effects on neurogenesis but still allowed for normal neurogenesis in many neuron-producing lineages.
Are there common mechanisms of neurogenesis used throughout an entire nervous system? We explored to what extent canonical proneural class I/II bHLH complexes are responsible for neurogenesis throughout the entire Caenorhabditis elegans nervous system. Distinct, lineage-specific proneural class II bHLH factors are generally thought to operate via interaction with a common, class I bHLH subunit, encoded by Daughterless in flies, the E proteins in vertebrates and HLH-2 in C. elegans. To eliminate function of all proneuronal class I/II bHLH complexes, we therefore genetically removed maternal and zygotic hlh-2 gene activity. We observed broad effects on neurogenesis, but still detected normal neurogenesis in many distinct neuron-producing lineages of the central and peripheral nervous system. Moreover, we found that hlh-2 selectively affects some aspects of neuron differentiation while leaving others unaffected. Although our studies confirm the function of proneuronal class I/II bHLH complexes in many different lineages throughout a nervous system, we conclude that their function is not universal, but rather restricted by lineage, cell type and components of differentiation programs affected.
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