New opportunities in integrative structural modeling

Integrative structural modeling enables structure determination of macromolecules and their complexes by integrating data from multiple sources. It has been successfully used to characterize macromolecular structures when a single structural biology technique was insufficient. Recent developments in cellular structural biology, including in-cell cryo-electron tomography and artificial intelligence-based structure prediction, have created new opportunities for integrative structural modeling. Here, we will review these opportunities along with the latest developments in integrative modeling methods and their applications. We also highlight open challenges and di-rections for further development.

Automated summary

Integrative structural modeling is a method that combines data from multiple sources to determine the structures of macromolecules and their complexes. Recent developments in cellular structural biology, such as in-cell cryo-electron tomography and artificial intelligence-based structure prediction, have opened up new opportunities for integrative structural modeling. This review discusses these opportunities, as well as the latest developments in integrative modeling methods and their applications, while also highlighting the challenges and directions for further development.