Response of Microbial Communities on Cathode with Different Potentials in a Single-Chamber Reactor

Microbes use both organic and inorganic compounds as electron donors, with different electronic potentials, to produce energy required for growth in environments. Conventional studies on the effects of different electron donors on microbial community has been extensively studied with a set cathode potential. However, it remains under-researched how a microbial community response to the different redox potentials in different environments. Here, we incubated a lake sediment in a single-chamber reactor equipped with three working electrodes, i.e., with potentials of - 0.29 V, - 0.05 V versus standard hydrogen electrode and open-circuit, respectively. Results reveal that the structure of bacterial communities was highly similar for all closed-circuit electrodes (- 0.29 V, - 0.05 V), while differing significantly from those on open-circuit electrodes. We also show that specific bacteria were preferentially enriched by different electrode potentials, i.e., Pseudomonas and Rhodobacter preferentially grew on - 0.05 V and - 0.29 V cathode potentials, Azospirillum and Bosea preferentially grew on - 0.05 V; while Ferrovibrio, Hydrogenophaga, Delftia, and Sphingobium preferentially grew on - 0.29 V. In addition, microorganisms selectively enriched on open-circuit electrodes possess higher connectivity and closer relationship than microorganisms selectively enriched on closed-circuit electrode.

Automated summary

Microbes can use both organic and inorganic compounds as electron donors to produce energy for growth. Previous studies have mainly focused on the effects of different electron donors on microbial community under a set cathode potential, while their response to different redox potentials in different environments remains under-researched. In this study, lake sediment was incubated in a reactor equipped with three working electrodes at different potentials. The results showed that the structure of bacterial communities was similar for closed-circuit electrodes, but significantly different for open-circuit electrodes. Specific bacteria were also preferentially enriched by different electrode potentials.