期刊
CNS NEUROSCIENCE & THERAPEUTICS
卷 29, 期 1, 页码 365-377出版社
WILEY
DOI: 10.1111/cns.14011
关键词
aging; HIV-1-tat; MAM-tethering; memory impairment; mitochondria-associated ER membranes; PTPIP51; VAPB
This study demonstrates that HIV-1 Tat protein disrupts mitochondrial-associated ER membranes (MAMs) through inducing PTPIP51 phosphorylation, leading to ROS accumulation, mitochondrial stress, and altered movement, therefore contributing to spatial memory impairment and premature brain aging.
IntroductionMitochondrial-associated ER membranes (MAMs) control many cellular functions, including calcium and lipid exchange, intracellular trafficking, and mitochondrial biogenesis. The disruption of these functions contributes to neurocognitive disorders, such as spatial memory impairment and premature brain aging. Using neuronal cells, we demonstrated that HIV-1 Tat protein deregulates the mitochondria. Methods& ResultsTo determine the mechanisms, we used a neuronal cell line and showed that Tat-induced changes in expression and interactions of both MAM-associated proteins and MAM tethering proteins. The addition of HIV-1 Tat protein alters expression levels of PTPIP51 and VAPB proteins in the MAM fraction but not the whole cell. Phosphorylation of PTPIP51 protein regulates its subcellular localization and function. We demonstrated that the Tat protein promotes PTPIP51 phosphorylation on tyrosine residues and prevents its binding to VAPB. Treatment of the cells with a kinase inhibitor restores the PTPIP51-VAPB interaction and overcomes the effect of Tat. ConclusionThese results suggest that Tat disrupts the MAM, through the induction of PTPIP51 phosphorylation, leading to ROS accumulation, mitochondrial stress, and altered movement. Hence, we concluded that interfering in the MAM-associated cellular pathways contributes to spatial memory impairment and premature brain aging often observed in HIV-1-infected patients.
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