Effect and mechanism of peanut skin proanthocyanidins on gliadin-induced Caco-2 celiac disease model cells

Proanthocyanidins have been shown to inhibit the signaling pathways related to oxidative stress and inflammation, also improved cell membrane integrity. The effect of peanut skin proanthocyanidins (PSPc) on CD remains unknown. In this paper, the effect and mechanism of PSPc on glial protein-induced Caco-2 cytotoxicity were studied. The results showed that PSPc may inhibit oxidative stress in DPG-induced CD model in vitro by regulating SIRT1/NRF2 pathway. By regulating SIRT1 and I kappa B signaling pathways, inhibit the phosphorylation of NF-kappa B and the deacetylation of NF-kappa B, inhibit inflammatory response, reduce release of inflammatory cy-tokines (IL-1 beta, IL-6, TNF-alpha), the cell survival rate was and the expression of TGM2 were improved, avoiding the damage of cell monolayer model. This experiment proved the prominent effect of PSPc on CD intervention. Studying the mechanism of PSPc in the treatment of CD injury will contribute to explore new therapies for CD which will be of great significance to supplement or replace gluten-free diets.

Automated summary

Peanut skin proanthocyanidins (PSPc) have a significant effect on CD intervention by inhibiting oxidative stress through regulation of the SIRT1/NRF2 pathway.