4.7 Article

Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biophysics

Rapid detection of miRNA via development of consecutive adenines (polyA)-based electrochemical biosensors

Xiaoyu Hua et al.

Summary: This study presents a rapid electrochemical detection method for miRNA let-7a using the polyA-H probe, with signal amplification through LAMP and i-motif structure formation. The total detection time is short and the sensitivity is high.

BIOSENSORS & BIOELECTRONICS (2022)

Article Chemistry, Multidisciplinary

Matching Capacitors to Self-Powered Biosensors for Signal Amplification: Toward Ultrasensitive Electrochemical Detection for MicroRNA-21-Triggered Catalytic Hairpin Assembly

Fu-Ting Wang et al.

Summary: This study investigates the use of a capacitor matched with a self-powered electrochemical biosensor for ultrasensitive detection of microRNA-21. The biosensor combines enzymatic biofuel cells and biological amplification technologies, with the capacitor joined circuit providing instant current for rapid detection. The matching capacitor significantly improves the detection sensitivity, and the developed biosensor shows a wide linear range and high sensitivity, making it promising for ultrasensitive electrochemical biosensing.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2022)

Article Chemistry, Analytical

Charge-Transfer Resonance and Surface Defect-Dominated WO3 Hollow Microspheres as SERS Substrates for the miRNA 155 Assay

Lingling Jiang et al.

Summary: In this study, a three-dimensional WO3 hollow microsphere was developed as a SERS-active substrate, and the influence of surface defects and charge transfer on SERS performance was investigated. The WO3 hollow microspheres showed a smaller band gap and rich surface defects, leading to strong charge transfer interaction and significant SERS enhancement. A SERS biosensor based on these semiconductor materials was constructed for the sensitive detection of miRNA 155, providing a new strategy for biomolecular analysis and disease diagnosis.

ANALYTICAL CHEMISTRY (2022)

Article Chemistry, Analytical

Isothermal and enzyme-free MicroRNA assay based on catalytic hairpin assembly and rare earth element labeled probes

Jiawei Shen et al.

Summary: This study proposes an isothermal and enzyme-free miRNA assay based on CHA amplification and ICP-MS detection. By utilizing hybridization reaction and CHA cycles, high sensitivity and specificity analysis of miRNA-141 were achieved. The method also demonstrated multiple detecting capability and the potential of becoming a high-throughput method.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Chemistry, Analytical

Multiregion Linear DNA Walker-Mediated Ultrasensitive Electrochemical Biosensor for miRNA Detection

Tong-Lin Hou et al.

Summary: In this study, an intelligent multiregion linear DNA walker (MLDW) was developed for ultrasensitive detection of miRNA. By using the rolling-circle amplification mediated by target miRNA, the MLDW achieved a significantly improved reaction rate. The reaction could be completed within 30 minutes, which is at least 4 times faster than traditional DNA walkers. As a proof of concept, the high-efficiency MLDW was applied to an electrochemical biosensing platform and successfully detected target miRNA-21 with a detection limit of 36 aM. This innovative design of DNA walker provides new insights for constructing functional DNA nanodevices and promoting the research of nucleic acid signal amplification.

ANALYTICAL CHEMISTRY (2022)

Review Chemistry, Inorganic & Nuclear

Application of electrochemical biosensors for the detection of microRNAs (miRNAs) related to cancer

Masoud Negahdary et al.

Summary: Molecular diagnoses, especially using miRNAs as cancer biomarkers, have shown great potential in cancer diagnosis and prognosis. Electrochemical biosensors have been widely researched for their ability to detect miRNAs, and nanomaterials have played an important role in optimizing the performance of these biosensors. This review provides an overview of the latest research on electrochemical biosensing of miRNAs, including the use of nanomaterials, different types of miRNAs, diagnostic techniques, and signal transducers.

COORDINATION CHEMISTRY REVIEWS (2022)

Article Nanoscience & Nanotechnology

Dual-Modal FexCuySe and Upconversion Nanoparticle Assemblies for Intracellular MicroRNA-21 Detection

Xiaoqian Jiang et al.

Summary: Utilizing DNA-driven FexCuySe@ upconversion nanoparticle core@satellite nanostructures, this study successfully developed a method for detecting miR-21 with high sensitivity and specificity. The FexCuySe@UCNP probes displayed dual UCL and MRI signals, showing a good linear relationship between the dual signals and miR-21 expression levels. The LOD for luminescence intensity was 0.0058 amol/ng(RNA) and for MRI signal was 0.0182 amol/ng(RNA), indicating the potential of this method for intracellular miR-21 detection.

ACS APPLIED MATERIALS & INTERFACES (2021)

Article Chemistry, Multidisciplinary

Simultaneous photoelectrochemical detection of dual microRNAs by capturing CdS quantum dots and methylene blue based on target-initiated strand displaced amplification

Jiafu Chang et al.

Summary: A novel PEC biosensor was proposed for highly sensitive and simultaneous detection of microRNA-21 and let-7a based on SDA reaction, achieving detection limits of 6.6 fmol/L and 15.4 fmol/L, respectively. The biosensor showed acceptable results in the simultaneous analysis of miRNA-21 and let-7a in breast cancer patient's serum, suggesting potential value in the diagnosis of miRNA-related diseases.

CHINESE CHEMICAL LETTERS (2021)

Article Nanoscience & Nanotechnology

Dual-Mode Aptasensor Assembled by a WO3/Fe2O3 Heterojunction for Paper-Based Colorimetric Prediction/Photoelectrochemical Multicomponent Analysis

Jianli Sun et al.

Summary: A bimodal photoelectrochemical (PEC) sensing platform based on DNA structural switching was designed for simultaneous detection of MUC1 and miRNA-21. The platform utilized WO3/Fe2O3 heterojunction material to enhance sensitivity and reduce background signal response, offering a wide dynamic range for multiple analytes. The method also introduced a visual colorimetric assay for real-time detection of targets.

ACS APPLIED MATERIALS & INTERFACES (2021)

Article Biophysics

Surface plasmon resonance imaging-based biosensor for multiplex and ultrasensitive detection of NSCLC-associated exosomal miRNAs using DNA programmed heterostructure of Au-on-Ag

Wenwen Wu et al.

Summary: The developed SPRi-based biosensor using DNA programmed Au-on-Ag heterostructure and DTF demonstrates a wide detection range, ultra-low limit of detection, enhanced capture efficiency, and accurate discrimination of NSCLC patients based on exosomal miRNA detection results, providing a promising tool for early diagnosis of NSCLC.

BIOSENSORS & BIOELECTRONICS (2021)

Article Biophysics

CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics

Richard Bruch et al.

Summary: MicroRNAs have become important biomarkers for various diseases in clinical and point-of-care diagnostics, but require consideration of other factors for accurate diagnosis. This study successfully designed and implemented multiplexed versions of an electrochemical microfluidic biosensor for simultaneous quantification of multiple miRNAs.

BIOSENSORS & BIOELECTRONICS (2021)

Article Chemistry, Multidisciplinary

An electrochemical biosensor based on DNA nano-bridge for amplified detection of exosomal microRNAs

Jing Zhang et al.

Summary: An electrochemical biosensor based on DNA nano-bridge was developed for detecting exosomal miR-21 with high sensitivity and selectivity, showing potential application in early diagnosis of cancer.

CHINESE CHEMICAL LETTERS (2021)

Article Chemistry, Analytical

Spatially localized amplification reaction with accelerated target conversion for sensitive microRNA detection

Min Qing et al.

Summary: An ingenious spatially localized amplification reaction (SLAR) was constructed by colocalizing the entropy-driven reaction (EDR) in a nanometer space, greatly accelerating target conversion for sensitive microRNA-21 (miRNA-21) detection. The SLAR assay exhibited excellent analytical performance with a detection limit of 6 pM and good specificity in distinguishing miRNA-21 from similar miRNAs, showing great promise for sensitive biomarker detection in early diagnosis.

TALANTA (2021)

Article Chemistry, Analytical

Multiplexed Detection and Imaging of Intracellular mRNAs Using a Four-Color Nanoprobe

Wei Pan et al.

ANALYTICAL CHEMISTRY (2013)

Review Chemistry, Multidisciplinary

MicroRNA: Function, Detection, and Bioanalysis

Haifeng Dong et al.

CHEMICAL REVIEWS (2013)

Article Chemistry, Multidisciplinary

Fabrication of a Structure-Specific RNA Binder for Array Detection of Label-Free MicroRNA

Jeong Min Lee et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)

Article Nanoscience & Nanotechnology

Programming the detection limits of biosensors through controlled nanostructuring

Leyla Soleymani et al.

NATURE NANOTECHNOLOGY (2009)

Article Biochemical Research Methods

MicroRNA detection by northern blotting using locked nucleic acid probes

Eva Varallyay et al.

NATURE PROTOCOLS (2008)

Article Multidisciplinary Sciences

Circulating microRNAs as stable blood-based markers for cancer detection

Patrick S. Mitchell et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Review Oncology

MicroRNA signatures in human cancers

George A. Calin et al.

NATURE REVIEWS CANCER (2006)

Review Oncology

Oncomirs - microRNAs with a role in cancer

A Esquela-Kerscher et al.

NATURE REVIEWS CANCER (2006)

Article Biochemical Research Methods

Amperometric detection in TMB/HRP-based assays

P Fanjul-Bolado et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2005)