期刊
CHEMOSPHERE
卷 308, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2022.136558
关键词
Synthetic phytohormone; Chlorella pyrenoidosa; Mariculture wastewater; Lipid accumulation; Key enzymes activity
资金
- National Natural Science Founda- tion of China [52070173]
- Qingdao Science and Technology Benefit People Demonstration and Guidance Project [21-1-4-sf-12-nsh]
- Fundamental Research Funds for the Central Universities [201964005]
- Urban and Rural Housing Construction Science and Technology Project of Shandong [2021-K8-7]
Synthetic phytohormones were used to enhance the growth and lipid accumulation of Chlorella pyrenoidosa, with acetyl-CoA carboxylase and malic enzyme being positively correlated with lipid accumulation.
Synthetic phytohormone (SP) is regarded as an attractive candidate for micmalgae cultivation due to its potential for high-value microalgae biomass production. Herein, alpha-naphthylacetic acid (NAA), indomethacin (IN) and 2,4-dichlorophenoxyacetic acid (2,4-D) were used for the mixotmphic cultivation of Chlorella pyrenoidosa with mariculture wastewater (MW) acidogenic fermentation effluent. The growth and lipid accumulation of Chlorella pyrenoidosa added with SP were enhanced, given their high bioavailability of the nutrients. Among these three SPs, IN was optimal for Chlorella pyrenoidosa growth, with the maximum optical density of 1.81. NAA exhibited the best performance for lipid production and the proportion of lipid reached 50.24%. Furthermore, the energy of Chlorella pyrenoidosa cultured with SP preferentially allocated to lipogenesis. To understand the mechanism of lipid accumulation in Chlorella pyrenoidosa in response to SP, the enzyme activities involved in carbon metabolism were determined. The malic enzyme (ME) and acetyl-CoA carboxylase (ACCase) were positively correlated with lipid accumulation. Phosphoenolpyruvate carboxylase (PEPC) was the negative feedback enzyme for lipid synthesis. The findings could provide valuable information for regulation mechanism of lipid accumulation and value-added products recovery by microalgae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据