A hormesis-like effect of FeS on heterotrophic denitrification and its mechanisms

To alleviate the insufficiency of carbon source in sewage, many sulfur-containing inorganic electron donors were added into traditional heterotrophic denitrification process. However, the effects of extraneous inorganic elec-tron donors on heterotrophic denitrification were still largely unknown. In this study, a hormesis-like effect of ferrous sulfide (FeS, a representative inorganic electron donors) on Paracoccus denitrificans was observed. Total nitrogen (TN) removal efficiency of P. denitrificans rose by 15% with the increase of FeS dosage from 0 to 0.3 g L-1 (low level), whereas the TN removal significantly decreased to 53% as the dosage of FeS mounted up to 5.0 g L-1 (high level). Furthermore, the impacts of FeS on glucose utilization and bacterial growth exhibited hormesis-like effects. A subsequent mechanistic study revealed that above influences were caused by its released ions (Fe2+, Fe3+, and S2-) rather than particle size. Further study illustrated that low dosage of FeS released a small amount of Fe2+ and Fe3+, which provided sufficient electrons via promoting glucose utilization, then improved denitrification. Conversely, FeS with high dosage inhibited denitrification via its released S2-, which suppressed the activity of key denitrifying enzymes rather than influenced glucose metabolism and electron provision. Our results provide an insight into improving denitrification efficiency of the mixotrophic process coexisting with autotrophic and heterotrophic denitrifiers.

Automated summary

To address the shortage of carbon source in sewage, inorganic electron donors containing sulfur were added to the traditional heterotrophic denitrification process. However, the effects of these extraneous inorganic electron donors on heterotrophic denitrification were largely unknown. This study discovered a hormesis-like effect of ferrous sulfide (FeS, an inorganic electron donor) on Paracoccus denitrificans, where the removal efficiency of total nitrogen increased with low dosage of FeS but decreased with high dosage. The study also revealed that the influence of FeS on glucose utilization and bacterial growth exhibited a hormesis-like effect.