A New Ceramide from Cissus Aralioides Baker (Vitaceae) and its Antimicrobial Activity

Purification through repeated column chromatography over silica gel and Sephadex LH-20 of the ethanol extract of the stems of Cissus aralioides (Baker) Planch. resulted in the isolation of a new ceramide, aralioidamide A (1), along with five known compounds (2-6). Their structures were determined by the extensive analyses of their spectroscopic (1D and 2D NMR) and spectrometric data, and comparison with those reported in the literature. Aralioidamide A (1) displayed weak antibacterial activity (MIC=256 mu g/mL) against Bacillus subtilis, Staphylococcus aureus and Shigella flexneri and was inactive (MIC>256 mu g/mL) against the tested fungi.

Automated summary

A new ceramide, aralioidamide A (1), and five known compounds (2-6) were isolated from the ethanol extract of the stems of Cissus aralioides (Baker) Planch. through repeated column chromatography over silica gel and Sephadex LH-20. The structures of these compounds were determined by extensive spectroscopic (1D and 2D NMR) and spectrometric analysis, as well as comparison with reported data. Aralioidamide A (1) exhibited weak antibacterial activity (MIC=256 mu g/mL) against Bacillus subtilis, Staphylococcus aureus, and Shigella flexneri, and was inactive (MIC>256 mu g/mL) against the tested fungi.