Acute toxic effects of new synthetic cannabinoid on brain: Neurobehavioral and Histological: Preclinical studies

The psychoactive effects of new synthetic cannabinoids (SCs), MDMB-4en-PINACA, are being marketed as a blend of herbs and spices. This study aims to determine the behavioral, neurochemical, histopathological, and immunohistochemical alterations associated with the acute toxicity of MDMB-4en-PINACA compounds. Methods: Adult male albino rats were administered various toxic doses of the drug (1.5, 3, and 6 mg/kg), and behavioral studies were conducted 2 and 24 h later; animals were then sacrificed. Histopathological and neurochemical examinations were performed. Two hours after intraperitoneal. Results: Intraperitoneal injection of MDMB-4en-PINACA, horizontal movement, the number of stops, and mobility ratio were significantly impaired, along with coordination and balance. In addition, it led to a decline in spatial learning and memory, and neurotransmitter concentrations decreased significantly in a dose-dependent manner. Further examination of the cerebral cortex and hippocampus histopathology revealed pathological degeneration of small pyramidal cells. Conclusion: Thus, these findings revealed that MDMB-4en-PINACA interferes with hippocampal function and impairs cognitive performance, highlighting the cognitive risk associated with SC abuse.

Automated summary

The study aims to determine the acute toxicity effects of MDMB-4en-PINACA compounds on behavior, neurochemistry, histopathology, and immunohistochemistry. The results showed impaired motor skills and coordination, decreased spatial learning and memory, and a dose-dependent decrease in neurotransmitter concentrations after injection of MDMB-4en-PINACA. Histopathological examination revealed degeneration of small pyramidal cells in the cerebral cortex and hippocampus. This study highlights the cognitive risk associated with synthetic cannabinoid abuse.