期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 368, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2022.110225
关键词
Mixture effect; Immunotoxicity; Apoptosis; Cytokines; Nanomechanics
资金
- Croatian-Chinese bilateral project Endocrine disrupting mechanism of typical environmental pollutants-EmergeTox - Ministry of Science and Education, Republic of Croatia
- Ministry of Science and Technology of the People's Republic of China
- STIM-REI [KK.01.1.1.01.0003]
- European Union through the European Regional Development Fund-the Operational Programme Competitiveness and Cohesion [KK.01.1.1.01]
The use of nano-enabled products has many benefits, but it also raises concerns about the release of nanoparticles into the environment. This study evaluated the combined effect of silver (AgNP) and polystyrene nanoparticles (PSNP) on human macrophages, and the results showed increased negative effects such as apoptosis, oxidative stress, and mitochondrial dysfunction when the two types of nanoparticles were combined.
Increasing use of nano-enabled products provides many benefits in various industrial processes and medical applications, but it also raises concern about release of nanoparticles (NPs) into the environment and subsequent human exposure. While potential toxicity of individual NPs types has been well described in scientific literature, exposure and health-related effects of nanomixtures has been poorly described. This study aimed to evaluate the combined effect of silver (AgNP) and polystyrene NPs (PSNP) on the human macrophages. AgNP are one of the most commercialized NPs due to efficient antimicrobial activity, while PSNP are ubiquitous in terrestrial and aquatic environments due to plastic pollution and degradation of polystyrene-based products. Differentiated monocytic cell line THP-1 were used as an in vitro model of human macrophages. Multiple aspects of cellular response to AgNP-PSNP nanomixture were analyzed including cell death, induction of apoptosis, oxidative stress response, expression of pro-and anti-inflammatory cytokines, and nanomechanical properties of cells. NPs up-take was visualized by confocal microscopy and quantified using flow cytometry. Results show that nanomixture increased apoptosis and cell death, expression of IL-6, IL-8 and TNFa, oxidative stress and mitochondrial dysfunction in cells compared to AgNP and PSNP applied as single treatments, indicating mixture additive action. Anti-inflammatory cytokines IL1b, IL-4 and IL-10 were not affected by combined exposure compared to single NPs. Visualization of NPs uptake and internalization showed that AgNP and PSNP were localized mostly in cytoplasm, with small fraction of AgNP translocated into cell nuclei, which explain increased number of double -stranded DNA breaks following exposure of cells to AgNPs alone or in the mixture. Study outcomes represent clear warnings on the human co-exposure to AgNP and PSNP that needs to be implemented in risk assessment approaches towards toxic-free environment.
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