Small-molecule fluorogenic probes for mitochondrial nanoscale imaging

Mitochondria are inextricably linked to the development of diseases and cell metabolism disorders. Super-resolution imaging (SRI) is crucial in enhancing our understanding of mitochondrial ultrafine structures and functions. In addition to high-precision instruments, super-resolution microscopy relies heavily on fluorescent materials with unique photophysical properties. Small-molecule fluorogenic probes (SMFPs) have excellent properties that make them ideal for mitochondrial SRI. This paper summarizes recent advances in the field of SMFPs, with a focus on the chemical and spectroscopic properties required for mitochondrial SRI. Finally, we discuss future challenges in this field, including the design principles of SMFPs and nanoscopic techniques.

Automated summary

Mitochondria play an essential role in the development of diseases and cellular metabolism disorders. Super-resolution imaging is crucial for studying the ultrafine structures and functions of mitochondria. Small-molecule fluorogenic probes have unique properties that make them ideal for super-resolution imaging of mitochondria. This paper summarizes recent advances in small-molecule fluorogenic probes, focusing on their chemical and spectroscopic properties for mitochondrial imaging. Future challenges in this field, including the design principles of small-molecule fluorogenic probes and nanoscopic techniques, are also discussed.