Solid-state NMR - a complementary technique for protein framework characterization

Protein frameworks are an emerging class of biomaterial with medical and technological applications. Frameworks are studied mainly by X-ray diffraction or scattering techniques. Complementary strategies are required. Here, we report solid-state NMR analyses of a microcrystalline protein-macrocycle framework and the rehydrated freeze-dried protein. This methodology may aid the characterization of low-crystallinity frameworks.

Automated summary

Protein frameworks are emerging biomaterials with potential medical and technological applications. This study introduces a complementary strategy using solid-state NMR analysis to characterize low-crystallinity frameworks, specifically focusing on a microcrystalline protein-macrocycle framework and its rehydrated freeze-dried form.