Si-rhodamine fluorescent probe for monitoring of hypochlorous acid in the brains of mice afflicted with neuroinflammation

Neuroinflammation leads to a persistent oxidative stress in the brain, and is closely related to the pathology of various neurological disorders. Hypochlorous acid (HClO) is a reactive oxygen species (ROS) that, at high levels, can cause brain tissue damage and neurogenic apoptosis. Herein, we designed and synthesized a silicon-rhodamine (SiR)-based formohydrazide (FH)-containing fluorescent probe, denoted as SiR-FH, for sensing HClO. This probe showed good selectivity, rapid response and high sensitivity. SiR-FH was successfully used to detect endogenous and exogenous HClO in living cells. Moreover, SiR-FH realized real-time monitoring of change in HClO flux in the brains of mice with LPS-induced neuroinflammation. The probe provides a practical tool for the monitoring of oxidative stress related to neuroinflammation.

Automated summary

Neuroinflammation leads to oxidative stress in the brain and is linked to various neurological disorders. A fluorescent probe, SiR-FH, was developed for detecting HClO. This probe demonstrated high selectivity, rapid response, and sensitivity, successfully detecting endogenous and exogenous HClO in living cells. Real-time monitoring of HClO flux in the brains of mice with LPS-induced neuroinflammation was achieved using SiR-FH, providing a practical tool for studying oxidative stress related to neuroinflammation.