☆ 4.5 Article

Auxotrophs compromise cell growth and fatty acid production in Saccharomyces cerevisiae

BIOTECHNOLOGY JOURNAL (2023)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Review Biotechnology & Applied Microbiology

Recent advances in construction and regulation of yeast cell factories

Xue Jiao et al.

Summary: The past decade has seen significant progress in synthetic biology, particularly in the development of yeast cell factories for green and sustainable chemical production. This review focuses on the advances in yeast engineering for the production of plant natural products in the last 5 years, including different yeast chassis and emerging genome editing tools. The review also highlights metabolic regulation strategies and challenges in C1-based chemical bioproduction by engineered yeast, and summarizes the future prospects in creating efficient yeast cell factories.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Substrate-induced differential degradation and partitioning of the two tryptophan permeases Tat1 and Tat2 into eisosomes in Saccharomyces cerevisiae

Ryoga Ishii et al.

Summary: This study found that Tat2 is rapidly degraded in a Rsp5-Bul1-dependent manner upon the addition of tryptophan, phenylalanine, or tyrosine, while Tat1 is unaffected. The expression of a ubiquitination-deficient variant of Tat2 leads to a reduction in cell yield. Tryptophan addition causes the rapid dissociation of Tat2 from eisosomes.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast

Ruibing Chen et al.

Summary: Advances in synthetic biology enable efficient and safe synthesis of valuable natural products by microbial hosts. However, the role of cofactors in microbial production of natural products has been largely ignored. In this study, the supply and recycling of cofactors in yeast cells were engineered to achieve high-level production of phenolic acids.

NATURE CHEMICAL BIOLOGY (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica

Dan-Ni Wang et al.

Summary: This study successfully synthesized high-value astaxanthin using genetically engineered yeast. By optimizing enzyme expression and addressing key bottlenecks in the biosynthesis process, the production of astaxanthin was significantly increased. The study also analyzed the impact of astaxanthin biosynthesis on cell metabolism, providing important insights for the production of high-value carotenoids.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2022)

添加到收藏夹

Letter Microbiology

Expanding the neutral sites for integrated gene expression in Saccharomyces cerevisiae

Sijia Kong et al.

Summary: In this study, we identified 30 neutral sites in the Saccharomyces cerevisiae genome that do not affect cellular fitness using the efficient CRISPR-Cas9 technique. These neutral sites can be used for extensive metabolic engineering and chemical production.

FEMS MICROBIOLOGY LETTERS (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha

Wei Yu et al.

Summary: In this study, genomic neutral sites for gene integration were screened in O. polymorpha via CRISPR-Cas9 technique. 17 potential neutral sites were identified, with fatty alcohol synthetic pathway genes successfully integrated into two of them, resulting in the production of 4.5 mg/L fatty alcohols. This method of neutral sites screening shows promise for streamline metabolic engineering in O. polymorpha and potentially other eukaryotes as well.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2021)

添加到收藏夹

Article Agriculture, Multidisciplinary

Overproduction of α-Farnesene in Saccharomyces cerevisiae by Farnesene Synthase Screening and Metabolic Engineering

Junhua Wang et al.

Summary: This study aimed to maximize the flux of farnesyl diphosphate to farnesene biosynthesis in Saccharomyces cerevisiae. Through screening and genetic engineering, a high alpha-farnesene producing yeast strain was developed, reaching a production level of 10.4 g/L. Various strategies were employed, such as overexpressing key enzymes and optimizing gene expression, to achieve the significant improvement in farnesene production.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites

Mahsa Babaei et al.

Summary: This study expanded the EasyClone-MarkerFree vector toolkit for baker's yeast S. cerevisiae by adding eight additional intergenic integration sites. The integration efficiency at the new sites was above 80%, with similar or higher expression levels of green fluorescent protein compared to the original sites. Integration at the new locations did not affect cellular growth.

FEMS YEAST RESEARCH (2021)

添加到收藏夹

Review Chemistry, Medicinal

Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast

Erdem Carsanba et al.

Summary: Terpenoids, a class of plant natural products with industrial and pharmaceutical importance, can be sustainably produced through microbial production, particularly using engineered yeast like Saccharomyces cerevisiae. Fermentation strategies play a crucial role in increasing yield and productivity of these compounds.

PHARMACEUTICALS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Engineering cellular metabolite transport for biosynthesis of computationally predicted tropane alkaloid derivatives in yeast

Prashanth Srinivasan et al.

Summary: Microbial biosynthesis of plant natural products can provide valuable medicinal compounds. However, metabolite transport limitations pose challenges in reconstructing complex plant pathways in unicellular hosts. This study demonstrates that alleviating transport limitations in an engineered pathway can increase tropane alkaloid production and enable screening of useful derivatives. Incorporating plant transporters, cofactor regeneration mechanisms, and optimized growth conditions into a yeast platform significantly improves de novo production of hyoscyamine and scopolamine. Additionally, computational tools for biosynthetic pathway prediction can be used to produce diverse tropane alkaloid derivatives from simple precursors.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Efficient targeted mutation of genomic essential genes in yeast Saccharomyces cerevisiae

Shan Yang et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations

Anagha Krishnan et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

添加到收藏夹

Review Multidisciplinary Sciences

Advanced Strategies for Production of Natural Products in Yeast

Ruibing Chen et al.

ISCIENCE (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Xiaozhou Luo et al.

NATURE (2019)

添加到收藏夹

Article Multidisciplinary Sciences

Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids

Prashanth Srinivasan et al.

NATURE COMMUNICATIONS (2019)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived hydrocarbons

Yiming Zhang et al.

BIOTECHNOLOGY AND BIOENGINEERING (2018)

添加到收藏夹

Review Energy & Fuels

Barriers and opportunities in bio-based production of hydrocarbons

Yongjin J. Zhou et al.

NATURE ENERGY (2018)

添加到收藏夹

Article Biochemical Research Methods

Porting the synthetic D-glucaric acid pathway from Escherichia coli to Saccharomyces cerevisiae

Amita Gupta et al.

BIOTECHNOLOGY JOURNAL (2016)

添加到收藏夹

Review Biochemical Research Methods

Developing fermentative terpenoid production for commercial usage

Michael D. Leavell et al.

CURRENT OPINION IN BIOTECHNOLOGY (2016)

添加到收藏夹

Article Multidisciplinary Sciences

Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories

Yongjin J. Zhou et al.

NATURE COMMUNICATIONS (2016)

添加到收藏夹

Article Biochemical Research Methods

Porting the synthetic D-glucaric acid pathway from Escherichia coli to Saccharomyces cerevisiae

Amita Gupta et al.

BIOTECHNOLOGY JOURNAL (2016)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Metabolic engineering in the biotechnological production of organic acids in the tricarboxylic acid cycle of microorganisms: Advances and prospects

Xian Yin et al.

BIOTECHNOLOGY ADVANCES (2015)

添加到收藏夹

Article Multidisciplinary Sciences

Planar Mobius aromatic pentalenes incorporating 16 and 18 valence electron osmiums

Congqing Zhu et al.

NATURE COMMUNICATIONS (2014)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Malic enzyme activity is not the only bottleneck for lipid accumulation in the oleaginous fungus Mucor circinelloides

Rosa Amarilis Rodriguez-Frometa et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2013)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica

John Blazeck et al.

JOURNAL OF BIOTECHNOLOGY (2013)

添加到收藏夹

Article Genetics & Heredity

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

Per O. Ljungdahl et al.

GENETICS (2012)

添加到收藏夹

Article Chemistry, Multidisciplinary

Modular Pathway Engineering of Diterpenoid Synthases and the Mevalonic Acid Pathway for Miltiradiene Production

Yongjin J. Zhou et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform

Michael Dalgaard Mikkelsen et al.

METABOLIC ENGINEERING (2012)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Developing an Industrial Artemisinic Acid Fermentation Process to Support the Cost-Effective Production of Antimalarial Artemisinin-Based Combination Therapies

Jacob R. Lenihan et al.

BIOTECHNOLOGY PROGRESS (2008)

添加到收藏夹

Article Microbiology

Malic enzyme:: the controlling activity for lipid production?: Overexpression of malic enzyme in Mucor circinelloides leads to a 2.5-fold increase in lipid accumulation

Ying Zhang et al.

MICROBIOLOGY-SGM (2007)

添加到收藏夹

Article Multidisciplinary Sciences

Production of the antimalarial drug precursor artemisinic acid in engineered yeast

DK Ro et al.

NATURE (2006)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.